|
Ten species of Cordyceps species were collected throughout Kangwon province including Chuncheon Dongsanmyun KNU forest experiment from June to September, 1993. Collected Cordyceps species were identified as Cordyceps militaris, C. roseostromata, C. kyuskuensis, C. scarabaeicolar, Phytocordyceps ninchukiospora, C. nutans, Paecilomyces tenuipes, C. sphecocephala, Hymenostilbe odonatae, Torrubiella sp.. C. militoris, type species of Cordyceps species, was mainly formed on pupae of Lepidoptera and found after the rainy season around July. Fruiting body of C. roseostromata was morphobgically similar to those of C. militaris, but relatively small in size and they were also found on larva or pupa of Lepidoptera. Fruiting body of C. scarabaeieob was found on adult Scarabseidae specifically and collected fruiting bodes of C. kyuskuensis were on larva of moth. C. nutans and C. sphecocepkala had hock specificity on Hemiptera and Hymenoptera, respectively. Each species formed elliptical fertile part attach to the slim and carneous stalk and they were collected the most in specimen number through whole season of the summer. Ascospore of Phytocordyceps ninchukiospora on seed was characterized by two viable, multiseptate, fusiform units linked end-to-end by a long, filiform connective. Paecilomyces tenuipes, imperfect stage of the genus Cordyceps is multi-infective fungi that attack all stages of all groups of insects. Hymenostilbe odonatae attacks only adult Odonata and Torrubiella sp. formed on spider was difficult to collect because it was found the back side of leaf. As results of cultural test PDA medium showed the best mycetial growth. In the experiment of effect of the acidity inside of the media, C. militaris was good on pH 5, C. nutans and Pkytocrordyeeps ninchukiospora were good on pH 6 and Paecilomyces tenuipes was on pH 7 and C, scarabaeicola was on pH 9. All isolates tested showed the best mycelial growth at 20℃. Morphologically similar isolates were used to analyze protein banding pattern among and within species. As a result, C. militaris, C. roseostromata and C. kyushuensis were clustered into close species and C. scarabaeicola and Phytornrdyceps ninchukiospora were relatively distant from those species.
|